Effects of dietary supplementation of probiotic, Clostridium butyricum, on growth performance, immune response, intestinal barrier function, and digestive enzyme activity in broiler chickens challenged with Escherichia coli K88

Table 1 The composition and nutrients of basal diet^a

Ingredient	Content, %	Chemical composition	Content
Corn	55.23	CP, %	20.90
Soybean meal	30.67	ME, Mcal/kg	3.00
Wheat shorts	4.00	Calcium, %	1.00
Fish meal^b	3.00	Total P, %	0.65
Soybean oil^c	2.90	Available P, %	0.45
DL-Methionine	0.27	Methionine + cysteine, %	0.90
NaCl	0.27	Lysine, %	1.05
Limestone	1.33
Calcium phosphate	1.33
Vitamin-mineral premix^d	1.00

^aNutrient level of the diets was based on NRC (1994)
^bCrude protein content is 62.5% and metabolizable energy is 2.79 Mcal/kg
^cMetabolizable energy is 8.8 Mcal/kg
^dSupplied per kilogram of diet: vitamin A(retinyl acetate), 1,500 IU; cholecalciferol, 200 IU; vitamin E(DL-α-tocopheryl acetate), 10 IU; riboflavin, 3.5 mg; pantothenic acid, 10 mg; niacin, 30 mg; cobalamin, 10μg; choline chloride, 1,000 mg; biotin, 0.15 mg; folic acid, 0.5 mg; thiamine 1.5 mg; pyridoxine 3.0 mg; Fe, 80 mg; Zn, 40 mg; Mn, 60 mg; I, 0.18 mg; Cu, 8 mg; Se, 0.15 mg

ISSN: 2049-1891