Table 1 Primers for PCR amplifications of porcine MSRB3

Part fragment cloning

M1F

ACCAGCCACTCAACTACTGC

1152

61

M1R

ACTTCCACCAGCAGAGCTTC

M2F

TGGTTCTAATTGCCTCAAAGG

774

59

M2R

GCAATGTCAGGACACCCTC

M3F

AGCATCCAGACTATCCCAGAAG

619

59

M3R

TACACCTCGCCATATTGACTCA

3’-RACE

3 F1

GGGGTGGACAAGGAAGATAA

-

55

3 F2

AAAAGCTCCTCTTCTAATGC

-

55

5’-RACE

5R1

CTTTGAGGCAATTAGAACCAG

-

60

5R2

TACAGTTGCCTTACATTCC

-

60

5R3

CATAAAACTGCCCTCCTACT

-

-

5R4

CACAGGCTCGCAGGGCTACT

-

-

qRT-PCR

M4F (target)

CCTCAGGGTCATGTAGGGATAAA

155

60

M4R (target)

TCCAGGATCTTTGTGATGTGTATATTC

60

G1F (control)

AGGGCATCCTGGGCTACACT

166

60

G1R (control)

TCCACCACCCTGTTGCTGTAG

60

Polymorphism discovery for exons

M345F (exon 1)

CCGTGCCCAGGAATT

345

54

M345R (exon1)

CGGCTCAGGAAAGAGG

M278F (exon 2)

GTGTTCAGTTCCAGATAAAACC

278

53

M278R (exon 2)

ACCCCAGACCAGTGCC

M314F (exon 3)

GGGACTGGTCTGGTCATT

314

56

M314R (exon 3)

CGGATTCAGCGGTTGG

M308F (exon 4)

GCCCTTGAAAGATTTATTGG

308

52

M308R (exon 4)

CCGGGGAGGAACAGAA

M190F (exon 5)

TTCCCTTTGCAGGTCAG

190

59

M190R (exon 5)

CATAGCCCTGCTTTGAGA

M272F (exon 6)

GTATCTCCTCTGTTTTGGTTC

272

59

M272R (exon 6)

TCATTGTGCTTGTCTGTCC

M618F (exon 7)

CGTGGTTTCCATCGTTC

618

52

M618R (exon 7)

CAAGCACCTTCTGCCTC

M2F (exon 7)

TGGTTCTAATTGCCTCAAAGG

774

59

M2R (exon 7)

GCAATGTCAGGACACCCTC

M3F (exon 7)

AGCATCCAGACTATCCCAGAAG

619

59

M3R (exon 7)

TACACCTCGCCATATTGACTCA

M839F (exon 7)

GCTCCTCTTCTAATGCTTACT

839

54

M839R (exon 7)

GCAGCCTATGGCAAACT

Polymorphism discovery for promoter

M175F

GGCTTTGGAATGAGGTTT

175

53

M175R

TTAGACGCTGTGCTAGTTGT

M692F

AAACAACTAGCACAGCGTC

692

50

M692R

GCCCAAATGCCAAAA

M694F

TTTCAGATACCCAGCATTG

694

50

M694R

GCCTGAAGGGGAGTGTTA

M754F

CCCATTACTGTGAGGAAAA

754

53

M754R

AAAGCAGAGCCGAGCA

M423F

TGGCTCGCTGTCGGA

423

58

M423R

GGCGGGCTCATGGAA