An efficient strategy for generation of transgenic mice by lentiviral transduction of male germline stem cells in vivo

Table 1 Percentage of eGFP positive pups obtained from F1 and F2 generations in Experiment1

Genetarion	Groups		No. litters	No. positive pups	Percentage
F1 generation	Pre-founder age	Injection methods			Success rate, %
	7 d	IS group A	31	21	67.8
	7 d	ST group B	60	40	66.7
	28 d	IS group C	45	30	66.7
	28 d	ST group D	57	40	70.2
	Grand total		193	131	67.88
F2 generation	Mating				Transmission rate, %
	Transgenic × Wild type		15	7	46.78
	Transgenic × Transgenic		21	13	61.90
	Grand total		36	20	55.56

IS injection of lentivirus into inter-tubular spaces, ST injection of lentivirus into seminiferous tubules

ISSN: 2049-1891