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Table 1 Percentage of eGFP positive pups obtained from F1 and F2 generations in Experiment1

From: An efficient strategy for generation of transgenic mice by lentiviral transduction of male germline stem cells in vivo

Genetarion Groups No. litters No. positive pups Percentage
F1 generation Pre-founder age Injection methods    Success rate, %
7 d IS group A 31 21 67.8
ST group B 60 40 66.7
28 d IS group C 45 30 66.7
ST group D 57 40 70.2
Grand total 193 131 67.88
F2 generation Mating    Transmission rate, %
Transgenic × Wild type 15 7 46.78
Transgenic × Transgenic 21 13 61.90
Grand total 36 20 55.56
  1. IS injection of lentivirus into inter-tubular spaces, ST injection of lentivirus into seminiferous tubules