Fig. 2From: An efficient strategy for generation of transgenic mice by lentiviral transduction of male germline stem cells in vivo Generation of transgenic mice via injection of lentivirus with EF1 promoter to drive eGFP expression in Experiment 1. a. Lentiviral vector used for generating transgenic mice. b-c. Recombinant lentiviruses injection into mouse testis via seminiferous tubules and inter-tubular spaces. d-e. Phenotype identification showing all germline transmission of the transgene such as pedigree analysis from pre-founder mice 006 (e) and 001 (d) with different ages or injection areas. gDNA amplification using the primers for eGFP transgene or GAPDH as shownBack to article page