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Fig. 2 | Journal of Animal Science and Biotechnology

Fig. 2

From: An efficient strategy for generation of transgenic mice by lentiviral transduction of male germline stem cells in vivo

Fig. 2

Generation of transgenic mice via injection of lentivirus with EF1 promoter to drive eGFP expression in Experiment 1. a. Lentiviral vector used for generating transgenic mice. b-c. Recombinant lentiviruses injection into mouse testis via seminiferous tubules and inter-tubular spaces. d-e. Phenotype identification showing all germline transmission of the transgene such as pedigree analysis from pre-founder mice 006 (e) and 001 (d) with different ages or injection areas. gDNA amplification using the primers for eGFP transgene or GAPDH as shown

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