Table 2 Primers for qPCR assay
Target species | Primer sequence (5’→3’)a | Amplicon |
|---|---|---|
Total bacteria | F: CGGTGAATACGTTCYCGG | 123 |
R: GGWTACCTTGTTACGACTT | ||
Methanogens | F: TTCGGTGGATCDCARAGRGC | 140 |
R: GBARGTCGWAWCCGTAGAATCC | ||
Protozoans | F: GCTTTCGWTGGTAGTGTATT | 223 |
R: CTTGCCCTCYAATCGTWCT | ||
F. succinogenes | F: GTTCGGAATTACTGGGCGTAAA | 121 |
R: CGCCTGCCCCTGAACTATC | ||
R. flavefaciens | F: GATGCCGCGTGGAGGAAGAAG | 286 |
R: CATTTCACCGCTACACCAGGAA | ||
R. albus | F: GTTTTAGGATTGTAAACCTCTGTCTT | 270 |
R: CCTAATATCTACGCATTTCACCGC | ||
B. fibrisolvens | F: TAACATGAGAGTTTGATCCTGGCTC | 135 |
R: CGTTACTCACCCGTCCGC |