In vitro antibacterial activity of thymol and carvacrol and their effects on broiler chickens challenged with Clostridium perfringens

Table 2 16s rDNA real-time PCR primers used to quantify intestinal bacteria

Target	Primer sequence (5′–3′) ^a	Amplicon size (bp)	Reference
Total bacteria	F:ACTCCTACGGGAGGCAGCAGT	200	[19]
Total bacteria	R:GTATTACCGCGGCTGCTGGCAC	200	[19]
Lactobacillus subgroup	F:AGCAGTAGGGAATCTTCCA	341	[19]
Lactobacillus subgroup	R:CACCGCTACACATGGAG	341	[19]
Escherichia subgroup ^b	F:GTTAATACCTTTGCTCATTGA	340	[20]
Escherichia subgroup ^b	R:ACCAGGGTATCTAATCCTGT	340	[20]
Clostridium perfringens	F:AAAGATGGCATCATCATTCAAC	279	[21]
Clostridium perfringens	R:TACCGTCATTATCTTCCCCAAA	279	[21]

^a F means forward, R means reverse
^b The targeted Escherichia subgroup contained genera of E. coli, Hafnia alvei and Shigella

ISSN: 2049-1891