In vitro antibacterial activity of thymol and carvacrol and their effects on broiler chickens challenged with Clostridium perfringens

Table 1 Diet composition and nutrient levels

Item (%, unless otherwise indicated)	Starter diets (day 0–21)	Grower diets (day 22–28)
Ingredient
Wheat	62.75	68.5
Soybean meal	29.61	23.72
Soybean oil	3.40	4.00
Dicalcium phosphate	1.91	1.63
Limestone	1.04	0.96
Sodium chloride	0.35	0.35
Choline chloride (50 %)	0.25	0.25
L-Lysine (99 %)	0.25	0.24
DL-Methionine (98 %)	0.19	0.11
Antioxidants	0.03	0.03
Trace mineral premix ^a	0.20	0.20
Vitamin premix ^b	0.02	0.02
Calculated nutrient levels
Metabolizable energy (Mcal/kg)	2.90	2.98
Protein	21.00	19.00
Calcium	1.00	0.90
Available phosphorus	0.45	0.40
Lysine	1.15	1.00
Methionine	0.50	0.40

^a The trace mineral premix provided the following (per kilogram of diet): manganese, 100 mg; zinc, 75 mg; iron, 80 mg; copper, 8 mg; selenium, 0.25 mg; iodine, 0.35 mg
^b The vitamin premix provided the following (per kilogram of diet): vitamin A, 18750 IU; vitamin D₃, 3750 IU; vitamin E, 28 IU; vitamin K₃, 3.975 mg; thiamine mononitrate, 3 mg; riboflavin, 9 mg; vitamin B₁₂, 0.0375 mg; d-biotin, 0.150 mg; folic acid, 1.875 mg; d-calcium pantothenate, 18 mg; nicotinic acid, 75 mg

ISSN: 2049-1891