Table 1 Primers used for gene cloning and expression analysis
Primer name | Oligo sequences (5′–3′) | Type |
|---|---|---|
cCYPF | GTCTGTGTGCCATGTGCTGTACGG | Complete ORF |
cCYPR | GTTTGTCGGGGAGGAGGATGAGGT | |
5′Router | GAACTTGCGGGCCATGATGT | 5′RACE |
5′Rinner | CCCGCAGCCGGACGACC | |
3′Router | CAAGCACTTCAAGGGGCTGAGCTT | 3′RACE |
3′Rinner | GACCAAGCGGGCAGTGGAAGTTGGGACCA | |
eCYP-F | TGCTGCAGGACTTTGTGG | Expression profile |
eCYP-R | TGGAGAGGATGCCCATGT | |
GAPDH-F | GGTGGTGCTAAGCGTGTCAT | Expression profile |
GAPDH-R | CCCTCCACAATGCCAAAGTT |