Influences of dietary protein sources and crude protein levels on intracellular free amino acid profile in the longissimus dorsi muscle of finishing gilts

Table 1 Ingredient and nutrient content of diets, as-fed %

Item	CSM12^a	CSM14^a	SBM12^a	SBM14^a
Ingredients
Corn	83.19	74.75	86.02	79.90
Wheat bran	3.30	5.40	1.75	2.70
Soybean meal	-	-	8.86	14.60
Cottonseed meal	8.15	13.72	-	-
Soybean oil	1.60	2.77	-	-
Limestone	1.06	1.15	0.98	1.04
Dicalcium phosphate	0.48	0.30	0.60	0.45
Sodium chloride	0.35	0.35	0.35	0.35
L-Lysine HCl	0.61	0.53	0.44	0.26
L-Threonine	0.21	0.17	0.15	0.07
L-Tryptophane	0.06	0.04	0.05	0.02
DL-Methionine	0.10	0.06	0.08	0.03
L-Valine	0.12	0.05	0.06	-
L-Isoleucine	0.17	0.13	0.08	-
L-Histidine	0.02	-	-	-
Choline chloride (50 %)	0.08	0.08	0.08	0.08
Vitamin-mineral premix^b	0.50	0.50	0.50	0.50
Analyzed nutrient composition^c
Gross energy, Mcal/kg	3.86	3.95	3.78	3.79
Crude protein	11.82	13.88	12.31	14.34
Arginine	0.85	1.17	0.67	0.75
Histidine	0.32	0.39	0.34	0.37
Isoleucine	0.36	0.43	0.46	0.51
Leucine	1.15	1.24	1.33	1.39
Lysine	1.00	0.97	0.86	0.82
Methionine	0.33	0.30	0.28	0.28
Phenylalanine	0.55	0.67	0.58	0.63
Threonine	0.58	0.59	0.57	0.55
Tryptophan	0.15	0.16	0.15	0.16
Valine	0.68	0.72	0.69	0.67
Sulfur-containing amino acids^d	0.62	0.60	0.54	0.57
Calculated nutrient composition^e
Metabolizable energy, Mcal/kg	3.30	3.30	3.30	3.30
Calcium	0.52	0.52	0.52	0.52
STTD phosphorus^f	0.21	0.21	0.21	0.21

^aCMS12, cottonseed meal as protein source, 12 % crude protein level; CMS14, cottonseed meal as protein source, 14 % crude protein level; SBM12, soybean meal as protein source, 12 % crude protein level; SBM14, soybean meal as protein source, 14 % crude protein level
^bThe vitamin-mineral premix provided the following quantities of vitamins and micro minerals per kilogram of complete diet: vitamin A as retinyl acetate, 4,000 IU; vitamin D₃ as cholecalciferol, 1,000 IU, vitamin E as DL-alpha tocopheryl acetate, 10 IU; vitamin K₃ as menadione nicotinamide bisulfite, 1.25 mg; thiamine as thiamine mononitrate, 0.5 mg; riboflavin, 2.1 mg; pyridoxine as pyridoxine hydrocloride, 1 mg; vitamin B₁₂, 0.007 mg; D-pantothenic acid as D-calcium pantothenate, 6 mg; niacin as nicotinamide and nicotinic acid, 12 mg; folic acid, 0.25 mg; biotin, 0.02 mg; Cu, 10 mg as copper sulfate; Fe, 75 mg as iron sulfate; I, 0.025 mg as potassium iodate; Mn, 10 mg as manganese sulfate; Se, 0.02 mg as sodium selenite; and Zn, 45 mg as zinc oxide
^cAnalytical results obtained according to AOAC [17]
^dSum of methionine and cysteine
^eCalculated values according to NRC [8]
^fSTTD = standardized total tract digestible

ISSN: 2049-1891