Partially replacing cornstarch in a high-concentrate diet with sucrose inhibited the ruminal trans-10 biohydrogenation pathway in vitro by changing populations of specific bacteria

Table 4 Composition of the medium- and long-chain fatty acid (FA) after 6 h in vitro incubation

Fatty acid, % of total FA	Sucrose level				SEM ¹	P-value²
	0	3 %	6 %	9 %	SEM ¹	T	L	Q
C12:0	0.48	0.58	0.42	0.67	0.058	0.507	0.456	0.563
C13:0 anteiso	0.36	0.25	0.34	0.29	0.024	0.409	0.569	0.622
C13:0	0.21	0.18	0.26	0.23	0.015	0.324	0.321	0.970
C14:0 iso	0.71	0.57	0.56	0.69	0.033	0.214	0.825	0.044
C14:0	2.62	2.28	2.19	2.62	0.090	0.218	0.879	0.044
C14:1 trans-9	2.21	1.83	2.12	2.19	0.087	0.367	0.783	0.209
C15:0 anteiso	3.12	2.53	2.33	3.15	0.167	0.228	0.946	0.047
C14:1 cis-9	1.85	1.61	1.42	1.65	0.073	0.271	0.219	0.122
C15:0	1.23	1.02	1.11	1.40	0.078	0.333	0.375	0.124
C16:0 iso	1.22	1.04	1.01	1.12	0.040	0.126	0.785	0.022
C16:0	27.6	25.1	25.8	26.1	0.492	0.294	0.366	0.158
C16:1 trans-9	0.53	0.49	0.46	0.48	0.021	0.771	0.395	0.551
C17:0 anteiso	1.47	1.24	1.15	1.50	0.073	0.271	0.982	0.060
C16:1cis-9	0.69	068	0.63	0.80	0.034	0.391	0.358	0.218
C17:0	0.58	0.63	0.53	0.61	0.018	0.251	0.958	0.630
C18:0 iso	0.05	0.06	0.12	0.11	0.015	0.261	0.077	0.896
C20:0	0.45	0.53	0.47	0.55	0.021	0.229	0.151	1.000
C20:1 trans-11	0.19	0.20	0.21	0.20	0.007	0.771	0.576	0.396
C22:1 trans-13	0.04	0.05	0.05	0.05	0.004	0.470	0.225	0.526
C24:0	0.36	0.60	0.50	0.65	0.051	0.176	0.089	0.648
C26:0	0.13	0.18	0.14	0.20	0.020	0.632	0.388	0.909

¹ SEM standard error of mean
² Probability of a significant effect of treatment (T), or linear (L) and quadratic (Q) orthogonal contrasts for dietary sucrose levels

ISSN: 2049-1891