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Table 3 Rumen pH, volatile fatty acid (VFA) profiles and ammonia-N concentration after 6 h in vitro incubation

From: Partially replacing cornstarch in a high-concentrate diet with sucrose inhibited the ruminal trans-10 biohydrogenation pathway in vitro by changing populations of specific bacteria

Item Sucrose level SEM1 P-value2
0 3 % 6 % 9 % T L Q
Rumen pH 6.17 6.17 6.18 6.19 0.014 0.971 0.641 1.000
Total VFA, mmol/L 73.8 74.8 75.9 76.5 0.391 0.056 0.009 0.738
Individual VFA (mol/100 mol)
 Acetate 63.0a 62.8b 62.7b 63.1a 0.044 0.003 0.380 <0.001
 Propionate 20.4a 20.1b 19.9bc 19.7c 0.074 0.001 <0.001 0.324
 Butyrate 12.2c 12.7b 13.1a 12.9ab 0.090 <0.001 <0.001 <0.001
 Isobutyrate 1.16a 1.14b 1.11c 1.08d 0.009 <0.001 <0.001 0.715
 Valerate 1.25b 1.29ab 1.30a 1.31a 0.008 0.015 0.002 0.457
 Isovalerate 2.07a 2.01b 1.95c 1.89d 0.018 <0.001 <0.001 0.804
A:P ratio3 3.09c 3.12bc 3.16ab 3.19a 0.012 0.002 <0.001 0.987
Ammonia, mg/dL 28.9a 27.5b 26.8b 26.6b 0.291 0.005 <0.001 0.114
  1. Note: Means within the same row with different superscripts differ significantly (P < 0.05)
  2. 1 SEM = standard error of mean
  3. 2 Probability of a significant effect of treatment (T), or linear (L) and quadratic (Q) orthogonal contrasts for dietary sucrose levels
  4. 3 A:P ratio = acetate to propionate ratio