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Table 2 Primers used for real-time PCR

From: Partially replacing cornstarch in a high-concentrate diet with sucrose inhibited the ruminal trans-10 biohydrogenation pathway in vitro by changing populations of specific bacteria

Target species

Primer

Sequence (5′ → 3′)

Reference

General bacteria

Forward

CGGCAACGAGCGCAACCC

McSweeney and Denman [41]

 

Reverse

CCATTGTAGCACGTGTGTAGCC

Butyrivibrio fibrisolvens

Forward

ACCGCATAAGCGCACGGA

Stevenson and Weimer [42]

 

Reverse

CGGGTCCATCTTGTACCGATAAAT

Streptococcus bovis

Forward

TTCCTAGAGATAGGAAGTTTCTTCGG

Stevenson and Weimer [42]

 

Reverse

ATGATGGCAACTAACAATAGGGGT

Megasphaera elsdenii

Forward

AGATGGGGACAACAGCTGGA

Stevenson and Weimer [42]

 

Reverse

CGAAAGCTCCGAAGAGCCT

Propionibacterium acnes

Forward

GGGTTGTAAACCGCTTTCGCCTG

Shingfield et al. [43]

 

Reverse

TGCTTTCGATACGGGTTGAC