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Table 3 Effects of CO2 and temperature treatments on in vitro pH, concentration of ammonia nitrogen (mg/100 ml) and microbial crude (mg/100 mL) protein, gas and methane production at 24 and 48 h of wheat straw

From: The effect of increased atmospheric temperature and CO2 concentration during crop growth on the chemical composition and in vitro rumen fermentation characteristics of wheat straw

Index1, g/100 g Time, h CK CO2 TEM CO2 + TEM S.E.M P- Value2
pH 24 6.62 ± 0.01a 6.67 ± 0.01b 6.64 ± 0.01a 6.69 ± 0.01b 0.01 *
48 6.56 ± 0.01a 6.58 ± 0.01ab 6.56 ± 0.01a 6.61 ± 0.01b 0.01 *
NH3-N 24 3.34 ± 0.03a 3.22 ± 0.03b 3.24 ± 0.02b 3.24 ± 0.03b 0.30 *
(mg/100 mL) 48 5.17 ± 0.03a 4.60 ± 0.08b 4.71 ± 0.06b 4.55 ± 0.07b 0.08 *
MCP 24 8.29 ± 0.14a 7.13 ± 0.21b 8.31 ± 0.13a 7.09 ± 0.43b 0.21 *
(mg/100 mL) 48 10.46 ± 0.19a 7.93 ± 0.19b 8.63 ± 0.32b 7.72 ± 0.55b 0.36 *
Gas production (mL) 24 25.41 ± 0.71 25.78 ± 0.46 25.80 ± 1.25 23.51 ± 1.21 0.50 ns
48 76.38 ± 1.28a 68.16 ± 1.55bc 73.63 ± 2.24ab 66.44 ± 1.49c 1.41 *
Methane production (mL) 24 6.41 ± 0.10a 7.84 ± 0.59a 3.95 ± 0.58b 7.25 ± 0.69a 0.50 *
48 25.80 ± 1.15a 27.58 ± 1.38a 17.88 ± 0.77b 20.67 ± 0.21b 1.24 *
  1. a,b Values within a column with different superscript letters are significantly different (P < 0.05)
  2. 1VFA: volatile fatty acid. 2ns = non-significant (P > 0.05)