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Table 3 Effects of CO2 and temperature treatments on in vitro pH, concentration of ammonia nitrogen (mg/100 ml) and microbial crude (mg/100 mL) protein, gas and methane production at 24 and 48 h of wheat straw

From: The effect of increased atmospheric temperature and CO2 concentration during crop growth on the chemical composition and in vitro rumen fermentation characteristics of wheat straw

Index1, g/100 g

Time, h

CK

CO2

TEM

CO2 + TEM

S.E.M

P- Value2

pH

24

6.62 ± 0.01a

6.67 ± 0.01b

6.64 ± 0.01a

6.69 ± 0.01b

0.01

*

48

6.56 ± 0.01a

6.58 ± 0.01ab

6.56 ± 0.01a

6.61 ± 0.01b

0.01

*

NH3-N

24

3.34 ± 0.03a

3.22 ± 0.03b

3.24 ± 0.02b

3.24 ± 0.03b

0.30

*

(mg/100 mL)

48

5.17 ± 0.03a

4.60 ± 0.08b

4.71 ± 0.06b

4.55 ± 0.07b

0.08

*

MCP

24

8.29 ± 0.14a

7.13 ± 0.21b

8.31 ± 0.13a

7.09 ± 0.43b

0.21

*

(mg/100 mL)

48

10.46 ± 0.19a

7.93 ± 0.19b

8.63 ± 0.32b

7.72 ± 0.55b

0.36

*

Gas production (mL)

24

25.41 ± 0.71

25.78 ± 0.46

25.80 ± 1.25

23.51 ± 1.21

0.50

ns

48

76.38 ± 1.28a

68.16 ± 1.55bc

73.63 ± 2.24ab

66.44 ± 1.49c

1.41

*

Methane production (mL)

24

6.41 ± 0.10a

7.84 ± 0.59a

3.95 ± 0.58b

7.25 ± 0.69a

0.50

*

48

25.80 ± 1.15a

27.58 ± 1.38a

17.88 ± 0.77b

20.67 ± 0.21b

1.24

*

  1. a,b Values within a column with different superscript letters are significantly different (P < 0.05)
  2. 1VFA: volatile fatty acid. 2ns = non-significant (P > 0.05)