Skip to main content

Table 2 Effect of injection timing on embryo development and transgene expression

From: Optimized production of transgenic buffalo embryos and offspring by cytoplasmic zygote injection

Time point of injection

n

N

Cleavage (% ± SD)

Blastocyst development

EGFP+ Blastocysts

Net yield EGFP+

(% ± SD)a

(% ± SD)b

Blastocysts (% ± SD)c

No injection

3

71

59 (83.1 ± 2.6)

25 (35.2 ± 5.5)

0

0

7–8 hpi

3

116

100 (86.2 ± 3.6)

30 (25.9 ± 3.9)

19 (63.3 ± 5.9)d

16.4 ± 5.9

12–13 hpi

3

102

83 (81.4 ± 3.8)

23 (22.5 ± 1.4)

12 (52.2 ± 2.9)d, e

11.8 ± 2.9

18–19 hpi

3

78

62 (79.5 ± 5.5)

16 (20.5 ± 9.1)

6 (37.5 ± 2.9)e

7.7 ± 2.9

  1. n number of independent experiments;a proportion of embryos placed into IVC (N) that developed into D7 blastocysts (B) grade 1–3;b proportion of EGFP+ blastocysts out of total D7 blastocysts;c proportion of EGFP+ blastocysts out of N;d, e rows with different superscripts within a column differ P < 0.05 from non-injected control