From: Optimized production of transgenic buffalo embryos and offspring by cytoplasmic zygote injection
Time point of injection | n | N | Cleavage (% ± SD) | Blastocyst development | EGFP+ Blastocysts | Net yield EGFP+ |
---|---|---|---|---|---|---|
(% ± SD)a | (% ± SD)b | Blastocysts (% ± SD)c | ||||
No injection | 3 | 71 | 59 (83.1 ± 2.6) | 25 (35.2 ± 5.5) | 0 | 0 |
7–8 hpi | 3 | 116 | 100 (86.2 ± 3.6) | 30 (25.9 ± 3.9) | 19 (63.3 ± 5.9)d | 16.4 ± 5.9 |
12–13 hpi | 3 | 102 | 83 (81.4 ± 3.8) | 23 (22.5 ± 1.4) | 12 (52.2 ± 2.9)d, e | 11.8 ± 2.9 |
18–19 hpi | 3 | 78 | 62 (79.5 ± 5.5) | 16 (20.5 ± 9.1) | 6 (37.5 ± 2.9)e | 7.7 ± 2.9 |