Skip to main content

Table 2 Effect of injection timing on embryo development and transgene expression

From: Optimized production of transgenic buffalo embryos and offspring by cytoplasmic zygote injection

Time point of injection n N Cleavage (% ± SD) Blastocyst development EGFP+ Blastocysts Net yield EGFP+
(% ± SD)a (% ± SD)b Blastocysts (% ± SD)c
No injection 3 71 59 (83.1 ± 2.6) 25 (35.2 ± 5.5) 0 0
7–8 hpi 3 116 100 (86.2 ± 3.6) 30 (25.9 ± 3.9) 19 (63.3 ± 5.9)d 16.4 ± 5.9
12–13 hpi 3 102 83 (81.4 ± 3.8) 23 (22.5 ± 1.4) 12 (52.2 ± 2.9)d, e 11.8 ± 2.9
18–19 hpi 3 78 62 (79.5 ± 5.5) 16 (20.5 ± 9.1) 6 (37.5 ± 2.9)e 7.7 ± 2.9
  1. n number of independent experiments;a proportion of embryos placed into IVC (N) that developed into D7 blastocysts (B) grade 1–3;b proportion of EGFP+ blastocysts out of total D7 blastocysts;c proportion of EGFP+ blastocysts out of N;d, e rows with different superscripts within a column differ P < 0.05 from non-injected control