From: Optimized production of transgenic buffalo embryos and offspring by cytoplasmic zygote injection
DNA Concentration | n | N | Cleavage(% ± SD) | Blastocyst development | EGFP+ Blastocysts | Net yield EGFP+ |
---|---|---|---|---|---|---|
(% ± SD)a | (% ± SD)b | Blastocysts (% ± SD)c | ||||
0 ng/μL | 3 | 57 | 49 (86.0 ± 4.9) | 18 (31.6 ± 4.1)d | 0 | 0 |
5 ng/μL | 3 | 110 | 83 (75.5 ± 2.1) | 28 (25.5 ± 1.7)de | 8 (28.6 ± 5.6)d | 7.3 ± 1.6 |
20 ng/μL | 3 | 98 | 72 (73.5 ± 1.8) | 22 (22.4 ± 6.8)de | 15 (68.2 ± 5.8)e | 15.3 ± 3.7 |
50 ng/μL | 3 | 106 | 76 (71.7 ± 5.6) | 15 (14.2 ± 3.8)e | 12 (80.0 ± 18.0)e | 11.3 ± 3.8 |