Skip to main content
Fig. 1 | Journal of Animal Science and Biotechnology

Fig. 1

From: Handmade cloning: recent advances, potential and pitfalls

Fig. 1

Outline showing procedures for Handmade cloning (HMC) (Reprinted from Nagai et al. [33]). Figure shows (1) the preparation of cytoplast by removal of cumulus layer of in-vitro matured oocytes (2) removal of zona pellucida from oocyte by digestion with pronase (3) hand bisection of zona-free oocytes with a blade, hence the name hand-made cloning, to obtain cytoplast (4) selecting a cytoplast under stereomicroscope (5) reconstructing embryo by fusing a donor somatic cell with cytoplast generating a demi-clone embryo which is again fused with 2nd half cytoplast (generated by same procedure as 1st cytoplast) to obtain a full sized clone embryo that is activated and implanted at blastocyst stage into recipient animal

Back to article page