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Table 3 The subsequent embryonic development of vitrified-warmed mouse MII oocytes treated with melatonin followed by IVF

From: No effect of exogenous melatonin on development of cryopreserved metaphase II oocytes in mouse

Group

Melatonin concentration

No of oocytes used for IVF

No. of oocytes developed to

2-cell(%)a

Blastocyst(%)a

Fresh

0 mol/L

60

57(93.55 ± 2.54)b

51(84.78 ± 0.36)b

Vitrified

0 mol/L

58

35(60.53 ± 5.48)c

25(43.51 ± 12.61)c

Vitrified

10−7 mol/L

59

33(55.39 ± 8.02)c

23(36.93 ± 6.15)c

  1. 1,a Number of 2-cell or blastocyst/Number of oocytes used for in vitro fertilization (IVF)
  2. 2, Percentage data are presented as mean ± SEM from at least 3 replicates.b andc, denote significant differences (P < 0.05)
  3. 3, Melatonin treatment: the vitrified-warmed mouse MII oocytes were cultured for 1 h in M2 medium with different concentrations (0, 10−7 mol/L) of melatonin, respectively, then they were used for IVF. The fresh mouse MII oocytes were used as control