Table 1 PCR primers used for SYBR green Q-PCR analysis
From: No effect of exogenous melatonin on development of cryopreserved metaphase II oocytes in mouse
Gene name | Assay ID | Primer Seq (5'→3') | Product length, bp | Tm, °C |
|---|---|---|---|---|
Hsp70 (Hspa1b) | NM_010478 | F:TGTTCCAGTAGCCTGGGAAG | 165 | 58 |
R:CCACAAAACCTTAACATGGACA | ||||
Hsp90 (Hsp90aa1) | NM_010480 | F:AAGGCAGAGGCTGACAAGA | 212 | 58 |
R:AGGGGAGGCATTTCTTCAGT | ||||
Nrf2 (Nfe2l2) | NM_010902.3 | F:CAGTGCTCCTATGCGTGAA | 109 | 58 |
R:GCGGCTTGAATGTTTGTC | ||||
Hsf1 | NM_008296N.2 | F:GCTCTGGACCCATAATCTC | 122 | 58 |
R:CTCTTGCTTGACACGGAC | ||||
Bcl-xl | NM_001289716.1 | F:GACAAGGAGATGCAGGTATTGG | 124 | 58 |
R:TCCCGTAGAGATCCACAAAAGT | ||||
GAPDH | NM_008084.3 | F:CATGGCCTTCCGTGTTCCTA | 104 | 58 |
R:GCCTGCTTACCACCTTCTT |