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Table 1 The composition and nutrient content of the basal diet

From: Growth, digestive and absorptive capacity and antioxidant status in intestine and hepatopancreas of sub-adult grass carp Ctenopharyngodonidella fed graded levels of dietary threonine

Ingredients Content, g/kg Nutrient contentf Content, g/kg
Fish meal 68.0 Crude protein 280.6
Casein 30.0 Crude lipid 46.8
Gelatin 39.9 Available phosphorus 6.0
Crystalline AA mixa 146.4 n-3 10.0
Threonine premixb 50.0 n-6 10.0
Glycine premixc 100.0   
α-starch 280.0   
Corn starch 34.6   
Fish oil 22.8   
Soybean oil 18.9   
Mineral premixd 20.0   
Vitamin premixe 10.0   
Ca(H2PO4)2 22.9   
Choline chloride (500 g/kg) 6.0   
Microcrystalline cellulose 150.0   
Ethoxyquin (300 g/kg) 0.5   
  1. aAmino acid mix: lysine, 15.99 g; methionine, 8.18 g; tryptophan, 3.27 g; arginine, 11.80 g; histidine, 7.23 g; isoleucine, 11.82 g; leucine, 18.99 g; valine, 14.24 g; phenylalanine, 12.53 g; tyrosine, 10.00 g; glutamate, 32.32 g
  2. bThreonine premix: Per kilogram of threonine premix composition from diet 1 to 6 was as follows (g/kg): L-threonine 0 g, 51.02 g, 102.04 g, 153.06 g, 204.08 g, 255.10 g, and corn starch 1, 000.00 g, 948.98 g, 897.96 g, 846.94 g, 795.92 g, 744.90 g, respectively
  3. cGlycine premix: Per kilogram of glycine premix composition from diet 1 to 6 was as follows (g/kg): L-glycine 524.58 g, 508.66 g, 492.75 g, 476.83 g, 460.92 g, 445.00 g, and corn starch 475.42 g, 491.34 g; 507.25 g; 523.17 g; 539.08 g; 555.00 g, respectively
  4. dPer kg of mineral premix: FeSO4•H2O (300 g/kg Fe), 25.00 g; CuSO4•5H2O (250 g/kg Cu), 0.60 g; ZnSO4•7H2O (345 g/kg Zn), 4.35 g; MnSO4•H2O (318 g/kg Mn), 2.04 g; KI (50 g/kg I), 1.10 g; NaSeO3 (10 g/kg Se), 2.50 g; MgSO4•H2O (150 g/kg Mg), 230.67 g. All ingredients were diluted with corn starch to 1 kg
  5. ePer kg of vitamin premix: retinyl acetate (500, 000 IU/g), 0.80 g; cholecalciferol (500, 000 IU/g), 0.48 g; DL-α tocopherol acetate (500 g/kg), 20.00 g; menadione (230 g/kg), 0.22 g; cyanocobalamin (10 g/kg), 0.10 g; D-biotin (20 g/kg), 5.00 g; folic acid (960 g/kg), 0.52 g; thiamine hydrochloride (980 g/kg), 0.12 g; ascorhyl acetate (930 g/kg), 7.16 g; niacin (990 g/kg), 2.58 g; meso-inositol (990 g/kg), 52.33 g; calcium-D-pantothenate (900 g/kg), 2.78 g; riboflavin (800 g/kg), 0.99 g; pyridoxine hydrochloride (980 g/kg), 0.62 g. All ingredients were diluted with corn starch to 1 kg
  6. fCrude protein and crude lipid contents were measured value. Available phosphorus, n-3 and n-6 contents were calculated according to NRC (1993)