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Table 1 Primers utilized for quantitative real-time PCR analysis

From: Factors controlling nutrient availability to the developing fetus in ruminants

Target 1

Forward/revers primers, (5’→3’) 2

Length of amplicon, bp

GenBank accession No 3

SLC7A1

CCTAGCGCTCCTGGTCATCA

56

AF212146

 

GGGCGTCCTTGCCAAGTA

  

SLC7A2

GCAGAGCAGCGCTGTCTTT

62

XM_002698665

 

ACTGTCCAGAGTGACGATTTTCC

  

SLC7A5

GGTGAACCCTGGTACGAATTTAGT

64

NM_174613

 

TCCACGCTCGAGAGGTATCTG

  

SLC7A6

CATTTGTGAACTGCGCCTATGT

72

NM_001075937

 

CCAGGACCTTGGCATAAGTGA

  

SLC7A7

TCAGGCTTGCCCTTCTACTTCT

64

NM_001075151

 

GGAGCCAAAGAGGTCGTTTG

  

SLC7A8

GGCCATGATCCACGTGAAG

65

NM_001192889

 

GGGTGGAGATGCATGTGAAGA

  

SLC38A2

CAGCTATAGTTCCAACAGCGACTTC

77

NM_001082424

 

CATCGGCATAATGGCTTTTCA

  

SLC38A4

TGCTTCATGCTTACAGCAAAGTG

63

NM_001205943

 

CAGCCAGGCGTACCATGAG

  
  1. 1The amplification target.
  2. 2The forward and reverse DNA oligos used in the amplification of the target. Forward and reverse primers do not necessarily indicate the in vivo direction of transcription.
  3. 3The accession number to the ovine or bovine sequence that was used during primer design.
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Journal of Animal Science and Biotechnology

ISSN: 2049-1891