Table 1 Primer sequences for qRT-PCRs
From: Transcriptome responses to heat stress in hypothalamus of a meat-type chicken
Gene symbol | Accession no. | Forward (5′-3′) | Reverse (5′-3′) | Product size, bp |
|---|---|---|---|---|
DNAJC13 | XM_418787 | TGAAAGGAGCAGGTTTGGTGAT | GTGTGCAAGTGACGAGGTAAGG | 115 |
HSPCB | NM_206959 | CAGGCGCAGACATCTCCAT | TGACGACTCCCAGGCATACTG | 125 |
HMOX1 | NM_205344 | TCCCTCCACGAGTTCAAGCT | CGGAAAATAAACAGGAGCATAGACA | 114 |
TH | NM_204805 | GAGAGCCATGCTGAACCTGTT | GGGCTTTCGGCTGAGTCTT | 130 |
EDN3 | AB235921 | TTTCGGTGCTCTTGTTTGGAT | CGGTCCTTCTCTGTTGTTTTCAC | 110 |
GCH1 | NM_205223 | CAGGAACGCCTTACCAAACAA | CTGGTTGCCGTTTTACTGTTCA | 140 |
GPR23 | CR353562 | TCAGATCGGGACAAACAAAGAGA | CCGCACGAGAGCATACAAGA | 115 |
SDC1 | XM_419972 | TGTAGTGACAGAGGAGCCAGTTG | TGAAGCACCGAAGGGAAGTG | 135 |
PDK4 | CR387492 | AGTTCCATCAGAAAAGCCCAGAT | TCCTTGTGCCATTGTAGGGACTA | 110 |
ITGA8 | NM_205288 | TAGCCGTGGCAGAACCTTACA | CCATAGCCGGGACCTGATTT | 140 |
SFRP1 | NM_204553 | CCAGTTCCCACAGGATTATGTCT | CCTCCGACTTCATCTCGTTGTC | 116 |
β-actin | NM_205518 | GAGAAATTGTGCGTGACATCA | CCTGAACCTCTCATTGCCA | 132 |