Table 1 Primer sequences used in RT-PCR
From: Impact of source tissue and ex vivo expansion on the characterization of goat mesenchymal stem cells
Gene | Primer sequences (5’ → 3’) | Product size, bp | Anneal T, °C | Source |
|---|---|---|---|---|
GAPDH | F: TCATGACCACAGTCCATGCCATCACT | 253 | 60 | [35] |
R: GATGTCATCATATTTGGCAGGTTTCTCC | ||||
OCT4 | F: AGGTGTTCAGCCAAACGACTATCTG | 192 | 55 | [35] |
R: TCGGTTCTCGATACTTGTCCGCTT | ||||
NANOG | F: GCCGAGGAATAGCAATGG | 444 | 53 | F: AY786437.2 |
R: TACAAATCTTCAGGCTGTATGTTG | R: [36] | |||
SOX2 | F: TGCAGTACAACTCCATGACCAGCT | 281 | 55 | [35] |
R: GTAGTGCTGGGACATGTGAAGTCTG | ||||
CD90 | F: GCACCATGAACCCTACCATC | 241 | 55 | NM_001034765.1 |
(THY1) | R: TTGGTTCGGGAGCTGTATTC | |||
CD73 | F: CTGAGACACCCGGATGAGAT | 160 | 57 | NM_174129.3 |
(NT5E) | R: ACTGGACCAGGTCAAAGGTG | |||
CD105 | F: AGATGCCAACATCACACAGC | 129 | 60 | NM_001076397.1 |
(ENG) | R: TCCAGACGAAGGAAGATGCT | |||
CD45 | F: GGGAGGAGGGAAAGCAAACC | 146 | 60 | XM_005691061.1 |
(PTPRC) | R: GCAGCTCTTCCCCATTCCAG | |||
CD34 | F: AGGTGTGCTCCTTGCTCCT | 134 | 55 | NM_174009 |
(CD34) | R: GCCCATCTCTCTCAGGTCAG | Â | Â | Â |