Figure 4

Oil Red O staining of MSCs and fibroblasts differentiated using two methods of adipogenic induction. P5 9004 BM-MSC (A, E, I), 9003 BM-MSC (B, F, J), 9003 ASC (C, G, K) and 1014 EF (D, H, L) were differentiated by the Adipo I method (A-D), Adipo II method (E-H), or cultured in expansion medium (I-L). Differentiated adipocytes accumulated lipid droplets in the cytoplasm that stain red with Oil Red O. Cells cultured in control medium (I-L) and 9003 BM-MSC induced by Adipo II (F) did not yield lipid-filled adipocytes. Representative images are shown in phase contrast at 200X magnification. Scale bars represent 100 μm.