Figure 3

Chondrogenic differentiation of MSCs and fibroblasts. P5 MSCs from 9004 BM-MSC (A, B), 9003 BM-MSC (C, D), 9003 ASC (E, F) and 1014 EF (G, H) were cultured in chondrogenic differentiation or control medium, respectively and stained with Alcian Blue, which stains cartilage blue. Cellular condensation, as well as ridge and micromass formations that stain positive were observed in 9004 BM-MSC, 9003 BM-MSC, 9003 ASC and 1014 EF cultured in chondrogenic medium (A, C, E, G respectively). Some staining was observed in cells cultured in control medium, but cells generally remained in monolayer (B, D, F, H respectively). Representative images are shown in phase contrast at 40X magnification. Scale bars represent 500 μm.