Table 1 Methods of clinical analysis

Anion gap

Calculated

Sodium

ISE, diluted

Potentiometry*

Potassium

ISE, diluted

Potentiometry*

Chloride

ISE, diluted

Potentiometry*

Bicarbonate

PEPC/NADH-NAD+

Zero-order kinetic*

Inorganic phosphate

Phosphomolybdate/UV 340 nm

Endpoint*

Calcium

Schwarzenbach/UV 600 nm

Endpoint*

Urea nitrogen

Enzymatic: urease with GLDH

First-order kinetic*

Creatinine

Jaffe

First-order kinetic*

Glucose

Hexokinase

Endpoint*

Total protein

Biuret

Endpoint*

Albumin

Bromocresol green

Endpoint*

Globulin

Calculated

AST

Modified IFCC

Zero-order kinetic*

Creatine kinase

Modified IFCC

Zero-order kinetic*

Alkaline phosphatase

Modified IFCC

Zero-order kinetic*

GGT

Modified IFCC

Zero-order kinetic*

Bilirubin total

Diazo

Endpoint*

SDH-37

D-Fructose to D-Sorbitol/NADH/UV 340 nm

Zero-order kinetic