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Table 1 Methods of clinical analysis

From: Hematologic and biochemical reference intervals for specific pathogen free 6-week-old Hampshire-Yorkshire crossbred pigs

Analyte Method/Principle Reaction type
Anion gap Calculated  
Sodium ISE, diluted Potentiometry*
Potassium ISE, diluted Potentiometry*
Chloride ISE, diluted Potentiometry*
Bicarbonate PEPC/NADH-NAD+ Zero-order kinetic*
Inorganic phosphate Phosphomolybdate/UV 340 nm Endpoint*
Calcium Schwarzenbach/UV 600 nm Endpoint*
Urea nitrogen Enzymatic: urease with GLDH First-order kinetic*
Creatinine Jaffe First-order kinetic*
Glucose Hexokinase Endpoint*
Total protein Biuret Endpoint*
Albumin Bromocresol green Endpoint*
Globulin Calculated  
AST Modified IFCC Zero-order kinetic*
Creatine kinase Modified IFCC Zero-order kinetic*
Alkaline phosphatase Modified IFCC Zero-order kinetic*
GGT Modified IFCC Zero-order kinetic*
Bilirubin total Diazo Endpoint*
SDH-37 D-Fructose to D-Sorbitol/NADH/UV 340 nm Zero-order kinetic
  1. All analysis were carried out at 37°C.
  2. AST, aspartate aminotransferase; GGT, γ-glutamyltransferase; SDH-37, sorbitol dehydrogenase; IFCC, International Federation of Clinical Chemistry; ISE, Ion specific electrode.
  3. *Roche Diagnostics GmbH.