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Figure 1 | Journal of Animal Science and Biotechnology

Figure 1

From: A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification

Figure 1

Use of a dRT-PCR assay to efficiently detect HP-PRRSV in crude serum samples. (A) Amplification plots for dRT-PCR and cRT-PCR assays. Threshold was set at 0.01. (B) Amplicons from the dRT-PCR assays and controls were electrophoresed on 3.5% (w/v) agarose gels. The arrows indicate the 73-bp target bands for HP-PRRSV. A 1 μL of purified HP-PRRSV RNA (Lane 1), 1 μL of HP-PRRSV-containing serum (Lane 2) and 1 μL of PRRSV-negative serum (Lane 3) were added to a 25 μL dRT-PCR mixture, respectively. All assays were performed in triplicate.

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