Figure 3

Restricted maternal nutrition increases Akt phosphorylation postnatally but does not affect myostatin or follistatin protein expression. Semitendinosus muscle was analyzed for Akt (B & C), myostatin (mstn, D & E) and follistatin (fstn, F) by western blot. Tubulin was used as a loading control. Representative blots are shown (A). Data are presented as mean ± SEM. *P = 0.006. CON = lambs from control-fed ewes (100% NRC), OVER = lambs from overfed ewes (140% NRC), RES = lambs from restricted-fed ewes (60% NRC), n = 4 lambs/group. ND = not detectable.