Quantitative analysis of ruminal methanogenic microbial populations in beef cattle divergent in phenotypic residual feed intake (RFI) offered contrasting diets

Table 1 PCR primers used for SYBR green qRT-PCR analysis

Target Taxon	Primer¹		E²	Product size, bp	Reference
Target Taxon	Forward	Reverse	E²	Product size, bp	Reference
16 s V3³	CCTACGGGAGGCAGCAG	ATTACCGCGGCTGCTGG	2.00	194	Muyzer et al., 1993 [18]
Total methanogens	GGATTAGATACCCSGGTAGT	GTTGARTCCAATTAAACCGCA	1.96	173	Hook et al., 2009 [16]
Methanosphaera stadtmanae	CTTAACTATAAGAATTGCTGGAG	TTCGTTACTCACCGTCAAGATC	2.01	150	Zhou et al., 2009 [17]

ISSN: 2049-1891