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Table 3 Survival and developmental capacity after cryopreservation (as indicated by rates of re-expansion and hatching) of embryos cultured in CR2aa medium supplemented with serum (Control) or with serum plus trans -10, cis- 12 CLA (CLA), compared to non-frozen embryos from the same groups

From: Trans-10, cis-12 conjugated linoleic acid reduces neutral lipid content and may affect cryotolerance of in vitro- produced crossbred bovine embryos

Group

In vitro culture

Blastocyst

Re-expansion

Hatching

  

n

% (n)

% (n)

Control

Fresh

49

-

77.6 (38)a

CLA

Fresh

44

-

88.6 (39)a

Control

Frozen-thawed

93

34.4 (32)a

14.0 (13)b

CLA

Frozen-thawed

103

56.3 (58)b

16.5 (17)b

  1. a,bDifferent lower case letters in the same column indicate statistical difference by Chi-square test (P < 0.01).
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Journal of Animal Science and Biotechnology

ISSN: 2049-1891