Table 1 Sequence of primers and annealing temperature specific for each gene
Gene | Primer Sequence (5′ → 3’) | Annealing Temperature | Fragment Size | GenBank Accession Nº/Reference |
|---|---|---|---|---|
GAPDH* | F: CCAACGTGTCTGTTGTGGATCTGA | 58°C | 237 | [42] |
R: GAGCTTGACAAAGTGGTCGTTGAG | ||||
FASN | F – 5′ GCACCGGTACCAAGGTGGGC 3′ | 58°C | 171 | NM_001012669.1 |
R – 5′ CGTGCTCCAGGGACAGCAGC 3′ | ||||
ACACB | F – 5′ CGGTGGTGCAGTGGCTGGAG 3′ | 58°C | 254 | XM_867921.3 |
F – 5′ CAGGAGGACCGGGGGTCAGG 3′ | ||||
AGPAT1 | F – 5′ CCGGAAGCGCACTGGGGATG 3′ | 58°C | 170 | NM_177518.1 |
R – 5′ TGGGAACCTGGGCCTGCACT 3′ | ||||
PRDX1 | F – 5′ ATGCCAGATGGTCAGTTCAAG 3′ | 53°C | 224 | [42] |
R – 5′ CCTTGTTTCTTGGGTGTGTTG 3′ | ||||
HSP70.1 | F – 5′ AACAAGATCACCATCACCAACG 3′ | 59°C | 275 | NM_174550 |
R – 5′ TCCTTCTCCGCCAAGGTGTTG | ||||
BCL-2 | F– 5′ TGGATGACCGAGTACCTGAA 3′ | 53°C | 120 | XM_586976 |
R – 5′ CAGCCAGGAGAAATCAAACA 3′ |