Effect of Saccharomyces cerevisiae on alfalfa nutrient degradation characteristics and rumen microbial populations of steers fed diets with different concentrate-to-forage ratios

Table 2 Primers used in real-time PCR for the detection of rumen microorganism

Target bacterium	Primer sequence (5'-3')	References
Total bacteria	F, CGGCAACGAGCGCAACCC	[15]
Total bacteria	R, CCATTGTAGCACGTGTGTAGCC	[15]
Rumen fungi	F, GAGGAAGTAAAAGTCGTAACAAGGTTTC	[16]
Rumen fungi	R, CAAATTCACAAAGGGTAGGATGATT	[16]
Protozoa	F, GCTTTCGWTGGTAGTGTATT	[16]
Protozoa	R, CTTGCCCTCYAATCGTWCT	[16]
Ruminobacter ablus	F, TGTTAACAGAGGGAAGCAAAGCA	[17]
Ruminobacter ablus	R, TGCAGCCTACAATCCGAACTAA	[17]
Ruminococcus flavefaciens	F, TGGCGGACGGGTGAGTAA	[17]
Ruminococcus flavefaciens	R, TTACCATCCGTTTCCAGAAGCT	[17]
Butyrivibrio fibrisolvens	F, ACCGCATAAGCGCACGGA	[17]
Butyrivibrio fibrisolvens	R, CGGGTCCATCTTGTACCGATAAAT	[17]
Fibrobacter succinogenes	F, GCGGGTAGCAAACAGGATTAGA	[17],[18]
Fibrobacter succinogenes	R, CCCCCGGACACCCAGTAT	[17],[18]
Selenomonas ruminantium	F, CAATAAGCATTCCGCCTGGG	[17],[18]
Selenomonas ruminantium	R, TTCACTCAATGTCAAGCCCTGG	[17],[18]
Streptococcus bovis	F, CTAATACCGCATAACAGCAT	[19]
Streptococcus bovis	R, AGAAACTTCCTATCTCTAGG	[19]
Ruminobacter amylophilus	F, CAACCAGTCGCATTCAGA	[19]
Ruminobacter amylophilus	R, CACTACTCATGGCAACAT	[19]
Lactobacillus	F, AGCAGTAGGGAATCTTCCA	[20]
	R, CGCCACTGGTGTTCYTCCATATA

ISSN: 2049-1891