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Table 1 Primer sequences used in real-time PCR

From: Evaluation of steroidogenic capacity after follicle stimulating hormone stimulation in bovine granulosa cells of Revalor 200® implanted heifers

  

Sequences of primers (5′-3′)

Gene

Accession no.

Forward

Reverse

1 3β-HSD

NM_174343

CCACACCAAAGCTACGATGA

GCAAGCCAGTACTGCAGAGA

2 CYP11A1

NM_176644

AAGTTTGACCCAACCAGGTG

GTGGATGAGGAAGAGGGTCA

3 CYP19A1

NM_174305

CAACAGCAGAGAAGCTGGAAGACA

CACCCACAACAGTCTGGATTTCCCT

4 GAPDH

NM_001034034

GGGTCATCATCTCTGCACCT

GGTCATAAGTCCCTCCACGA

5 MRPL19

NM_001046068

GGAAAGCAGGTTCTTGAGTCC

TGGCATATGGGTCAGCAGTA

6 PPIA

XM_002690515

GGTACTGGTGGCAAGTCCAT

GCCATCCAACCACTCAGTCT

7 STAR

BC110213

CCCATGGAGAGGCTTTATGA

CGTGAGTGATGACCGTGTCT

  1. 13β-HSD = 3β-hydroxysteroid dehydrogenase.
  2. 2CYP11A1 = P450 side chain cleavage.
  3. 3CYP19A1 = aromatase.
  4. 4GAPDH = glyceraldehyde-3-phosphate dehydrogenase.
  5. 5MRPL19 = mitochondrial ribosomal protein L19.
  6. 6PPIA = cyclophilin A.
  7. 7STAR = steroidogenic acute regulatory protein.