Figure 2

Volume changes within bovine MII- stage oocytes during different CPA treatments. a) oocytes pre-treated with EG20 for 5 min and then transferred to pre-cooled EG40; b) oocytes pre-treated with EG20 for 5 min, transferred to pre-cooled (4°C) EG40 for 30 s, and then flushed with pre-cooled (4°C) Gly30; c) oocytes pre-treated with EG20 for 5 min, transferred to pre-cooled (4°C) EG40 for 30 s, and then flushed with pre-cooled (4°C) Gly40; d) oocytes pre-treated with EG20 for 5 min, transferred to pre-cooled (4°C) EG40 for 30 s, and then flushed with pre-cooled (4°C) Gly50; e) oocytes after pre-treatment with EG20 for 5 min and then transfer to pre-cooled Gly50.