Table 4 Forward and reverse porcine primer sequences and accession numbers of genes used in quantitative RT-PCR analysis
Gene | Forward primer | Reverse primer | GenBank accession # | cDNA size, bp |
|---|---|---|---|---|
CYP8b1 | 5′-aagtgggccggctccagtgt-3′ | 5′-gcccgagccccatggcatag-3′ | NM_214426.1 | 625 |
Aldh2 | 5′-gcatcggcatgttgcgccct-3′ | 5′-ggtaggtccggtcccgctca-3′ | NM_001044611.2 | 374 |
TST | 5′-cgggctcaagggcggtacct-3′ | 5′-tttgcccacggggcatggac-3′ | XM_001926303 | 435 |
Gstmu 5 | 5′-tcgcccgcaagcacaacatgt-3′ | 5′-acaagcagtgcaagtccgcct-3′ | AK233626.1 | 453 |
β-actin | 5′-acatcaaggagaagctgtgc-3′ | 5′-ttggcgtagaggtccttgc-3′ | AY550069 | 256 |