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Table 4 Quantitative RT-PCR of non-opsonized Salmonella strain Δ adhE:kan during invasion of THP-1 cells

From: Deletions in the pyruvate pathway of Salmonella Typhimurium alter SPI1-mediated gene expression and infectivity

Time post-infection Salmonella gene Expression* Regulation**
1 h All genes tested were significantly down-regulated
2.5 h All genes tested were significantly down-regulated
4 h hilC −2.53 DOWN
invC −2.63
invE −2.46
invF −2.14
invH −1.98
orgB −1.71
prgH −1.79
prgJ −1.67
prgK −1.91
rtsA −2.58
sicA −3.91
sicP −1.73
sipB −4.44
sipC −2.91
6 h hilA −2.08 DOWN
hilC −2.76
hilD −1.56
invC −2.76
invE −2.07
invF −2.44
invH −2.30
orgA −2.36
orgB −1.31
prgH −1.71
rtsA −2.33
sicA −3.31
sicP −1.72
sipA −2.22
sipB −3.58
sipC −2.28
  1. *Values listed are relative fold-change values as compared to the wild-type strain ATCC 14028; **All genes listed are significant at p-value < 0.05.