Deletions in the pyruvate pathway of Salmonella Typhimurium alter SPI1-mediated gene expression and infectivity

Table 4 Quantitative RT-PCR of non-opsonized Salmonella strain Δ adhE:kan during invasion of THP-1 cells

Time post-infection	*Salmonella* gene	Expression*	Regulation**
1 h	All genes tested were significantly down-regulated
2.5 h	All genes tested were significantly down-regulated
4 h	hilC	−2.53	DOWN
	invC	−2.63
	invE	−2.46
	invF	−2.14
	invH	−1.98
	orgB	−1.71
	prgH	−1.79
	prgJ	−1.67
	prgK	−1.91
	rtsA	−2.58
	sicA	−3.91
	sicP	−1.73
	sipB	−4.44
	sipC	−2.91
6 h	hilA	−2.08	DOWN
	hilC	−2.76
	hilD	−1.56
	invC	−2.76
	invE	−2.07
	invF	−2.44
	invH	−2.30
	orgA	−2.36
	orgB	−1.31
	prgH	−1.71
	rtsA	−2.33
	sicA	−3.31
	sicP	−1.72
	sipA	−2.22
	sipB	−3.58
	sipC	−2.28

*Values listed are relative fold-change values as compared to the wild-type strain ATCC 14028; **All genes listed are significant at p-value < 0.05.

ISSN: 2049-1891