Deletions in the pyruvate pathway of Salmonella Typhimurium alter SPI1-mediated gene expression and infectivity

Table 3 Quantitative RT-PCR of non-opsonized Salmonella strain Δ pflB::kan during invasion of THP-1 cells

Time post-infection	*Salmonella* gene	Expression*	Regulation**
1 h	No change
2.5 h	invE	1.71	UP
	invC	−1.46	DOWN
	invH	−1.41
	rtsA	−1.70
	sicA	−1.52
	sicP	−1.34
	sipC	−1.36
4 h	hilA	1.72	UP
	hilD	1.36
	invF	1.19
	prgJ	1.18
6 h	hilA	−1.61	DOWN
	hilC	−1.97
	hilD	−1.55
	invC	−1.54
	invE	−1.51
	invF	−1.52
	invH	−1.61
	orgA	−1.66
	orgB	−1.30
	prgH	−1.43
	prgJ	−1.46
	rtsA	−1.83
	sicA	−1.89
	sicP	−1.63
	sipA	−1.58
	sipB	−2.00
	sipC	−1.70

*Values listed are relative fold-change values as compared to the wild-type strain ATCC 14028; **All genes listed are significant at p-value < 0.05.

ISSN: 2049-1891