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Table 2 Quantitative RT-PCR of Salmonella during invasion of HCT-8 cells

From: Deletions in the pyruvate pathway of Salmonella Typhimurium alter SPI1-mediated gene expression and infectivity

Time post-infection

Strain

Salmonella gene

Expression*

Regulation**

1 h

ΔpflB::kan

hilC

1.28

UP

hilD

1.60

prgJ

1.48

prgK

1.92

sicA

1.31

ΔadhE::kan

hilC

1.26

invE

3.16

invH

1.56

orgB

1.25

prgJ

1.60

prgK

1.96

sicA

1.15

4 h

ΔpflB::kan

rtsA

2.52

UP

sicA

1.64

 

sipA

1.88

 

ΔadhE::kan

hilA

3.12

 

orgA

3.70

 

orgB

1.66

 

rtsA

2.47

 

sicA

1.67

 

sicP

1.56

 

sipA

1.62

 

18 h

ΔpflB::kan

hilD

2.15

UP

prgH

1.85

prgJ

3.08

sicA

2.78

sipA

1.88

sipC

3.21

ΔadhE::kan

invC

1.61

24 h

ΔpflB::kan

prgH

−1.92

DOWN

prgJ

−2.43

prgK

−1.75

sicP

−3.41

sipA

−3.36

sipB

−3.39

ΔadhE::kan

invC

−2.66

sipA

−2.07

  1. *Values listed are relative fold-change values as compared to the wild-type strain ATCC 14028; **All genes listed are significant at p-value < 0.05.