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Table 2 Quantitative RT-PCR of Salmonella during invasion of HCT-8 cells

From: Deletions in the pyruvate pathway of Salmonella Typhimurium alter SPI1-mediated gene expression and infectivity

Time post-infection Strain Salmonella gene Expression* Regulation**
1 h ΔpflB::kan hilC 1.28 UP
hilD 1.60
prgJ 1.48
prgK 1.92
sicA 1.31
ΔadhE::kan hilC 1.26
invE 3.16
invH 1.56
orgB 1.25
prgJ 1.60
prgK 1.96
sicA 1.15
4 h ΔpflB::kan rtsA 2.52 UP
sicA 1.64  
sipA 1.88  
ΔadhE::kan hilA 3.12  
orgA 3.70  
orgB 1.66  
rtsA 2.47  
sicA 1.67  
sicP 1.56  
sipA 1.62  
18 h ΔpflB::kan hilD 2.15 UP
prgH 1.85
prgJ 3.08
sicA 2.78
sipA 1.88
sipC 3.21
ΔadhE::kan invC 1.61
24 h ΔpflB::kan prgH −1.92 DOWN
prgJ −2.43
prgK −1.75
sicP −3.41
sipA −3.36
sipB −3.39
ΔadhE::kan invC −2.66
sipA −2.07
  1. *Values listed are relative fold-change values as compared to the wild-type strain ATCC 14028; **All genes listed are significant at p-value < 0.05.