Table 1 A list of the Salmonella primers used in this study
Gene | Forward sequence (5′-3′) | Reverse sequence (5′-3′) | Locus or reference | Technique |
|---|---|---|---|---|
pflB | GAAGGTAGGTGTTACATGTCCGAGCTT AATGAAAAGTTAGCCACAGTGCAGGCT GGAGCTGCTTC | TTTCAGTCAAACCCATTACAT GGTCTGCGTGAAGGTACGAGT AATCATATGAATATCCTCCTTAG | STM0973 | Lambda red |
adhE | TATCAGGAGAGCATTATGGCTGTTACT AATGTCGCTGAACTTAACGTGCAGGCT GGAGCTGCTTC | AAAACAGATAACGAATTAAGC GGATTTTTTCGCTTTTTTCTC TGCCATATGAATATCCTCCTTAG | STM1749 | |
16S rRNA | AGGCCTTCGGGTTGTAAAGT | GTTAGCCGGTGCTTCTTCTG | [49] | qPCR |
hilA | ATAGCAAACTCCCGACGATG | ATTAAGGCGACAGAGCTGG | [50] | |
hilC | CTCACCTCTTCAGCGGCCAGT | CACCCGCAAATGGTCACAGGCT | STM2867 | |
hilD | GGTAGTTAACGTGACGCTTG | GATCTTCTGCGCTTTCTCTG | [50] | |
invC | TCGGTCGATCGCTGCAA | CCCTGGTCGCGAAAATATTC | STM2894 | |
invE | CGAATGACGCCAGCTGTTC | TGCGTCAGGCGTCGTAAA | STM2897 | |
invF | TGAAAGCCGACACAATGAAAAT | GCCTGCTCGCAAAAAAGC | STM2899 | |
invH | GGTGCCCCTCCCTTCCT | TGCGTTGGCCAGTTGCT | STM2900 | |
invJ | CGCCGCCGGTTAATTG | GCTTCCGCTGCAACCAA | STM2892 | |
orgA | AGGCAGGGAGCCTTGCTT | CCCTGATGCATTGCCAAAA | STM2870 | |
orgB | ACCATCCCGAAACGCTTTTA | TTGCCCCTCAGGCTTATCG | STM2869 | |
prgH | TGAACGGCTGTGAGTTTCCA | GCGCATCACTCTGACCTACCA | STM2874 | |
prgJ | GGTCTATGGAAACGGACATTGTC | CGCCGAACCAGAAAAAGC | STM2872 | |
prgK | GGGTGGAAATAGCGCAGATG | TCAGCTCGCGGAGACGATA | STM2871 | |
rtsA | ACCCGTGGTGAGCTTGATGAGT | CCTGTCCAGGTGGGGAGCAT | SEE_03946 | |
sicA | GATGAGTCTCTGCGGGCAAA | GCTCTGTCTCCGCCGTTTT | STM2886 | |
sicP | AGATGATATCTGGTTATTGAACGGTATG | CTGCCGCCAGATAGAATCG | STM2879 | |
sipA | CGTCTTCGCCTCAGGAGAAT | TGCCGGGCTCTTTCGTT | STM2882 | |
sipB | GGCGCGCTGCTAACCAT | TCGCCCCACCGGTAAAA | STM2885 | |
sipC | ATCAGGCTGGTCGATTTACG | GTACGCCGCTACTCAGGAAC | [50] |