Effects of implant and intake level on phosphorylated mammalian target of rapamycin (phospho-mTOR) abundance in cultured myocytes. Myocytes from a non-study steer were treated with serum (20% of total media) from study steers drawn on d 0 and 28. In chart A, steers were either implanted (gray bars; IMPLANT) or not implanted (open bars; CONTROL) with a long-acting TBA:E2 implant; in chart B, steers were fed a common diet at either 2.0 (gray bars; HIGH) or 1.0 (white bars; LOW) × maintenance NE requirements. (Effect of intake P = 0.51; implant P < 0.01; intake × implant P = 0.88. Error bars represent pooled SEM = 0.134).