Effect of dietary nonphytate phosphorus on laying performance and small intestinal epithelial phosphate transporter expression in Dwarf pink-shell laying hens

Table 2 Oligonucleotide PCR primers used for the determination of vitamin D receptor protein and sodium phosphate type-IIb mRNA expression

Gene	GenBank accession	Orientation	Primer sequence (5′ → 3′)	Predicted size, bp
Sodium Phosphate type-IIb	NM_204474.1	Forward	CTTTTACTTGGCTGGCTGGAT	148
Sodium Phosphate type-IIb	NM_204474.1	Reverse	AGGGTGAGGGGATAAGAACG	148
Calbindin	NM_205513.1	Forward	AATCTGCGTTGCTTCCATACA	218
Calbindin	NM_205513.1	Reverse	CATTTAGTTGCCTGAGTTCACCT	218
Vitamin D receptor	NM_205098.1	Forward	GGCTCAGGTTTTGCAGATTTG	100
Vitamin D receptor	NM_205098.1	Reverse	CAGCATCGCCTTTCCCATT	100
β-actin	NM_205518.1	Forward	AACACCCACACCCCTGTGAT	100
β-actin	NM_205518.1	Reverse	TGAGTCAAGCGCCAAAAGAA	100

ISSN: 2049-1891