Effect of dietary nonphytate phosphorus on laying performance and small intestinal epithelial phosphate transporter expression in Dwarf pink-shell laying hens

Table 1 Composition of laying hens diets

Items	Dietary nonphytate phosphorus density, %
Items	0.20	0.25	0.30	0.35	0.40
Ingredient, %
Corn	60.49	60.31	60.12	59.95	59.75
Soybean meal	27.14	27.17	27.21	27.24	27.27
Soybean oil	0.97	1.03	1.09	1.15	1.21
Limestone	10.12	9.91	9.71	9.49	9.31
Dicalcium phosphate	0.47	0.77	1.06	1.36	1.65
Salt	0.3	0.3	0.3	0.3	0.3
Trace mineral premix¹	0.2	0.2	0.2	0.2	0.2
Vitamin premix²	0.02	0.02	0.02	0.02	0.02
DL-methionine	0.17	0.17	0.17	0.17	0.17
Choline chloride	0.12	0.12	0.12	0.12	0.12
Total	100	100	100	100	100
Nutrient composition, %
ME, Mcal/kg	2.7	2.7	2.7	2.7	2.7
CP	17	17	17	17	17
Calcium	3.75	3.75	3.75	3.75	3.75
Nonphytate phosphorus³	0.2	0.25	0.3	0.35	0.4
Lysine	0.86	0.86	0.86	0.86	0.86
Methionine + Cystine	0.65	0.65	0.65	0.65	0.65
Tryptophan	0.21	0.21	0.21	0.21	0.21
Threonine	0.66	0.66	0.66	0.66	0.66

¹Mineral premix provided per kg of diet: Mn, 100 mg; Fe, 80 mg; Zn, 75 mg; Cu, 8 mg; I, 0.35 mg; Se, 0.15 mg.
²Vitamin premix provided per kg of diet: vitamin A, 12,500 IU; vitamin D₃, 2,500 IU; vitamin E, 30 IU; vitamin K₃, 2.65 mg; vitamin B₁, 2 mg; vitamin B₂, 6 mg; vitamin B₁₂, 0.025 mg; biotin, 0.0325 mg; folic acid, 1.25 mg; pantothenic acid, 12 mg; niacin, 50 mg.
³Analyzed levels of nonphytate phosphorus were 0.19, 0.24, 0.31, 0.35, and 0.42 in each nonphytate phosphorus density diet.

ISSN: 2049-1891