Figure 1From: Hypoxia promotes cell proliferation by modulating E2F1 in chicken pulmonary arterial smooth muscle cellsEffect of hypoxia on the proliferation of chicken pulmonary arterial smooth muscle cells (PASMCs). PASMCs were exposed to normoxia (21% oxygen) or under different concentrations of hypoxia (1%, 2%, 3%, or 5% oxygen) for 24 h, cell viability (A) and DNA synthesis (B) were analyzed by using MTT assay and [3H]-thymidine incorporation assay respectively. Each value is mean ± SEM of five separate experiments, each performed in triplicate. (C) PASMCs were exposed to normoxia (21% oxygen) or hypoxia (2% oxygen) for indicated time points, cell viability was determined and expressed as mean ± SEM of five separate experiments, each performed in triplicate. Values for the normoxic cells at each time point were set as 100%, *P<0.05 compared with normoxic control.Back to article page