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Figure 1 | Journal of Animal Science and Biotechnology

Figure 1

From: Hypoxia promotes cell proliferation by modulating E2F1 in chicken pulmonary arterial smooth muscle cells

Figure 1

Effect of hypoxia on the proliferation of chicken pulmonary arterial smooth muscle cells (PASMCs). PASMCs were exposed to normoxia (21% oxygen) or under different concentrations of hypoxia (1%, 2%, 3%, or 5% oxygen) for 24 h, cell viability (A) and DNA synthesis (B) were analyzed by using MTT assay and [3H]-thymidine incorporation assay respectively. Each value is mean ± SEM of five separate experiments, each performed in triplicate. (C) PASMCs were exposed to normoxia (21% oxygen) or hypoxia (2% oxygen) for indicated time points, cell viability was determined and expressed as mean ± SEM of five separate experiments, each performed in triplicate. Values for the normoxic cells at each time point were set as 100%, *P<0.05 compared with normoxic control.

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