Table 1 Nucleotide sequences of specific primers
Target genes | GenBank accession No. | Products | |
|---|---|---|---|
Length (bp) | Primer sequences | ||
cGnRH-I | NM_001080877.1 | 202 | F:5′-GCTTGGCTCAACACTGGTCT-3′ |
R:5′-CTGGCTTCTCCTTCGATCAG-3′ | |||
Leptin R | AF169827 | 97 | F:5′-GCTTGCTCAGGTAGCTCCTG-3′ |
R:5′-TGCGGCACGTATGGCACGAT -3′ | |||
LH (β) | HQ872606.1 | 223 | F:5′-GTATGGCTGTGACCACCACGG-3′ |
R:5′-CTGCACGGTGCAGTCGGAG-3′ | |||
FSH (β) | NM_204257.1 | 142 | F:5′-CGTGGTGCTCAGGATAC-3′ |
R:5′-AAAAGATTCAGGATGGT-3′ | |||
β-actin | NM_205518.1 | 299 | F:5′-TGCGTGACATCAAGGAGAAG-3′ |
R:5′-TGCCAGGGTACATTGTGGTA-3′ |