Skip to main content

Advertisement

Table 1 Nucleotide sequences of specific primers

From: Sexual maturation in hens is not associated with increases in serum leptin and the expression of leptin receptor mRNA in hypothalamus

Target genes GenBank accession No. Products  
   Length (bp) Primer sequences
cGnRH-I NM_001080877.1 202 F:5′-GCTTGGCTCAACACTGGTCT-3′
R:5′-CTGGCTTCTCCTTCGATCAG-3′
Leptin R AF169827 97 F:5′-GCTTGCTCAGGTAGCTCCTG-3′
R:5′-TGCGGCACGTATGGCACGAT -3′
LH (β) HQ872606.1 223 F:5′-GTATGGCTGTGACCACCACGG-3′
R:5′-CTGCACGGTGCAGTCGGAG-3′
FSH (β) NM_204257.1 142 F:5′-CGTGGTGCTCAGGATAC-3′
R:5′-AAAAGATTCAGGATGGT-3′
β-actin NM_205518.1 299 F:5′-TGCGTGACATCAAGGAGAAG-3′
    R:5′-TGCCAGGGTACATTGTGGTA-3′