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Table 1 Sequences of the oligonucleotide primers used in real-time quantitative PCR

From: Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo

RNA target

Primer sequences

Size of PCR product, bp

 

Sense

Anti-sense

 

MHC class I*

5′-AAGAAGGGGAAGGGCTACAA-3′

5′-AAGCAGTGCAGGCAAAGAAT-3′

222

MHC class II*

5′-CTCGAGGTCATGATCAGCAA-3′

5′-TGTAAACGTCTCCCCTTTGG-3′

312

Interferon γ

5′-TGAGCCAGATTGTTTCGA-3′

5′-ACGCCATCAGGAAGGTTG-3′

118

LITAF

5′-TTCTATGACCGCCCAGTT-3′

5′-CAGAGCATCAACGCAAAA-3′

165

IL-2*

5′-CGGGATCCATGATGTGCAAAGTACTG-3′

5′-CGGTCGACTTATTTTTGCAGATATCT-3′

80

IL-4

5′-GAGAGGTTTCCTGCGTCAAG-3′

5′-TGACGCATGTTGAGGAAGAG-3′

76

IL-6

5′-ATAAATCCCGATGAAGTGG-3′

5′-CTCACGGTCTTCTCCATAAA-3′

146

Il-10

5′-CAATCCAGGGACGATGAAC-3′

5′-GCAGGTGAAGAAGCGGTGA-3′

94

Il-12

5′-GGGAACAGAACTGAAAGG-3′

5′-GCTGATAATCTCGTGGG-3′

109

H9N2

5′-ATGCGGTGGAAGATGGG-3′

5′-AGGCGACAGTCGAATAAATG-3′

198

GAPDH*

5′-CCTCTCTGGCAAAGTCCAAG-3′

5′-CATCTGCCCATTTGATGTTG-3′

200

  1. MHC = Major histocompatibility complex.
  2. GAPDH = glyceraldehyde-3-phosphate-dehydrogenase.