Table 2 Primers used for quantitative real time PCR
Primer | Sequence, 5′to 3′ | Melting temperature, Tm | Product length, bp |
|---|---|---|---|
SRY | F: CTAGAGAATCCCAAAATGAAAAACTC | 53°C | 150 |
R: ATATTTATAGCCCGGGTATTTGTCTC | 55°C | ||
DDX3Y | F: GTTAGATTTCTGCAAATACTTGGTGTT | 59°C | 101 |
R: GCATAGTGTCTTGTTCAATTATACGAC | 60°C | ||
USP9Y | F: GTACACAGTGGTCAAGCAAGTGGTG | 59°C | 178 |
R: CTTCTCCCATGTACTCTCCACCAAA | 58°C | ||
TSPY | F: AGTTGTGAGCCCAGTTGTCA | 52°C | 148 |
R: CACCTCCTCCACGATGTCTT | 54°C |