Table 1 Primers used to amplify the gene fragments
Primer | Sequence, 5′to 3′ | Melting temperature, Tm | Product length, bp |
|---|---|---|---|
SRY | F: CGCAGTGCAGTCGTATGCTTCTGC | 61°C | 736 |
R: GAGCGCCTTTGTTAGCGAGAGTAAGG | 61°C | ||
DDX3Y | F: TTCGCGCCTTTCTTCAGGCATGAGTCA | 61°C | 2010 |
R: CAGATTCAGTTGCCCCACCAGTCAAC | 61°C | ||
USP9Y | F: TGTGGGACTCAAAAATGCTGGTGCTAC | 60°C | 1646 |
R: ACTCCAGAAGGTATTCAGAGAAACGATTTGA | 59°C | ||
TSPY | F: ATGTCGCGTCCCTTCGCCTCTGC | 62°C | 1141 |
R: TCAGTTGTCTCTCATGGACGAACCTTCCT | 62°C |