Figure 2

Construction of standard curves for SRY , DDX3Y , USP9Y and TSPY quantification. The standard curves for SRY, DDX3Y, USP9Y and TSPY absolute copy number determination together with their respective line equations are shown here. Set of serial 10-fold dilutions was made for each gene. Each of these dilutions (standard dilutions) had known copies of the plasmid construct harboring the respective gene fragment as an insert. X-axis represents log transformed values of the standard copy number and Y-axis represents the crossing point (Cp) values i.e. the fractional cycle number required for the fluorescence to cross the threshold.