Skip to main content

Table 1 Primers used for detection of pluripotency marker genes

From: Efficient production of chimeric mice from embryonic stem cells injected into 4- to 8-cell and blastocyst embryos

Gene

Sequences

Length, bp

Oct4

CTG AGG GCC AGG CAG GAG CAC GAG

485

CTG TAG GGA GGG CTT CGG GCA CTT

Nanog

AGG GTC TGC TAC TGA GAT GCT

363

CAA CAC CTG GTT TTT CTG CCA CCG

Sox-2

GGT TAC CTC TTC CTC CCA CTC CAG

193

TCA CAT GTG CGA CAG GGG CAG

Rex-1

ACG AGT GGC AGT TTC TTC TTG GGA

287

TAT GAC TCA CTT CCA GGG GGC ACT

β-actin

CCG CCA CCA GTT CGC CAT G

778

 

CCG CTC GTT GCC AAT AGT GAT GAC