Figure 3

Generation of chimeric embryos and mice using the PMM method. Four- to eight-cell and blastocyst stage embryos were injected with 10–15 ES cells with the application of Piezo (A). The in vivo development of transgenic 4- to 8-cell stage embryos injected with ES cells was observed under the fluorescence microscope (B, black arrow: ICM). Chimeras were obtained from injecting the ES cells into the embryos (C). Scale bar = 20 μm.