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Table 1 Summary of commercialized qRT-PCR methods for the detection of miRNAs

From: Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling

Method

Forward primer

Reverse primer

Detection method

References

Stem-loop

specific

universal

Specific probe

[54]

Stem-loop

specific

universal

LNA UPL probe

[32]

Poly(A)

specific

universal

SYBR

[55–57]

Poly(A)

LNA specific

LNA specific

SYBR

[58]

  1. LNA: locked nucleic acid; UPL: Universal probe library; SYBR: SYBR Green I.
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Journal of Animal Science and Biotechnology

ISSN: 2049-1891